Composite

Part:BBa_K2598025

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-09-25)
Revision as of 08:59, 25 September 2018 by Registry (Talk | contribs)

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lacZ under CGG-regulated promoter pCGG

This part contains lacZ under a CGG-regulated promoter pCGG, T7 RNAP sigma fragment CGG(BBa_K2598011) can direct T7 core fragment to pCGG, trigering the expression of downstream genes. A strong terminator is added to avi=oid recombination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3112
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None