Coding

Part:BBa_K2656018

Designed by: Adrián Requena Gutiérrez, Carolina Ropero   Group: iGEM18_Valencia_UPV   (2018-09-23)
Revision as of 17:16, 30 September 2018 by Adrireq (Talk | contribs)


amilCP Coding Sequence

Part BBa_K2656021 is the amil Chromoprotein coding secuence BBa_K592009 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Braid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].

The characterization of this protein (and by extension of all the other part that codify for the sfGFP) has been performed with our transcriptional unit BBa_K2656113. This transcriptional unit was assembled in a Golden Braid alpha1 plasmid(REF) including the following parts:

  • BBa_K2656004: the J23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • BBa_K2656009: the B0030 ribosome biding site in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • BBa_K2656018: This part.
  • BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/chromoprotein
//function/reporter/pigment
Parameters
None