Translational_Unit
mRFP1
Part:BBa_K2835000:Design
Designed by: Emma Larsson, Hanna Norbäck Group: iGEM18_Stockholm (2018-08-15)
Enigneered mutant of mRFP1 + RBS (compatible with all iGEM RFC assembly standards)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.
Source
Discosoma striata (coral)