Coding

Part:BBa_K2586020:Design

Designed by: Janek Meiner   Group: iGEM18_Goettingen   (2018-09-13)
Revision as of 10:46, 13 September 2018 by Dschannek96 (Talk | contribs) (→‎Design Notes)


AroA*: Resistant aroA-variant in Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 346
    Illegal AgeI site found at 787
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1021


Design Notes

The part includes 2 point mutations: At position 287 a Guanin was changed to a Cytosin and at position 547 a Guanin was changed to an Adenin. This changes the original aroA gene, so that it acquires a resistance to the herbicide glyphosate.

Source

This part was ordered and used in PCR amplification experiments. The PCR product was used as insert to clone this part into the required EcoRI and PstI digested pSB1C3 backbone.


References