Coding

Part:BBa_K2586020:Design

Designed by: Janek Meißner   Group: iGEM18_Goettingen   (2018-09-13)
Revision as of 09:19, 13 September 2018 by Registry (Talk | contribs)

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AroA*: Resistant aroA-variant in Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 346
    Illegal AgeI site found at 787
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1021


Design Notes

The part includes 2 point mutations: yet to come


Source

This part was ordered and used in PCR amplification experiments. The PCR product was used as insert to clone this part into the required EcoRI and PstI digested pSB1C3 backbone.


References