Composite
Part:BBa_K2638013
Designed by: Erika Schneider Group: iGEM18_Bielefeld-CeBiTec (2018-09-02)
Weak constitutive expression system for CopC and CopD
Weak constitutive expression of CopC (BBa_K2638001) and CopD (BBa_K2638002) with weak anderson promoters J231015 and RBSs BBa_B0034 and a double terminator (BBa_K823017).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 566
Illegal NheI site found at 589 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 899
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 365
Illegal AgeI site found at 419 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 307
Illegal BsaI.rc site found at 885
[edit]
Categories
Parameters
None |