Composite

Part:BBa_K2486019

Designed by: Cauă Westmann, Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-24)
Revision as of 01:19, 2 November 2017 by Gabriellovate (Talk | contribs)


PlldR_tetR/LldR based lactate biosensor

A lactate biosensor composed by parts BBa_K2486007 and BBa_K24860010. The part BBa_K2486007 works as a LldR generator, one of the repressor proteins capable of blocking gene expression of our PlldR_tetR promoter (the other protein is TetR). The other componet of our biosensor is the part BBa_K24860010 a reporter module composed of our hybrid promoter (PlldR_tetR promoter) and a GFP::lva.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 874
    Illegal NheI site found at 897
    Illegal NheI site found at 1033
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1743


[edit]
Categories
Parameters
None