Composite

Part:BBa_K2423002

Designed by: iGEM Uppsala 2017   Group: iGEM17_Uppsala   (2017-08-18)
Revision as of 10:26, 27 October 2017 by Rixina (Talk | contribs)


UGTCs2 under the control of a constitutive promoter (BBa_J23106)

This part codes for a cytosolic glycosyltransferase found in Crocus sativus. The enzyme will catalyse the conversion of crocetin to α-crocin in the presence of UDP-glucose. It is a modified version of BBa_K1033112, with two stop codons and a SGGS linker for the His tag. BBa_K2423008 is a slightly modifed version of this part (it uses BBa_J04500 as a promoter and RBS instead).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 593
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 766
    Illegal AgeI site found at 1121
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None