Composite

Part:BBa_I712047:Design

Designed by: Katja Kolar   Group: iGEM07_Ljubljana   (2007-10-23)
Revision as of 16:58, 26 October 2007 by Katja (Talk | contribs) (→‎Design Notes)


CMVp - HA - CD4 - HIV protease cleavage site - T7RNAP - NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1307
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4783
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1920
    Illegal NgoMIV site found at 1969
    Illegal AgeI site found at 2050
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1754
    Illegal SapI.rc site found at 842


Design Notes

The construct was cloned into pSB1AK3 vector with terminator.

Source

References