Collections/Cell-Free TX-TL

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This year our team (Lethbridge Collegiate) has put all the protein components of a cell-free transcription-translation (TX-TL) system in BioBrick standard with affinity purification tags. The idea is to provide an easy to express and purify TX-TL system that anyone can perform and use for protein synthesis testing or teaching.

We sought to make all the TX-TL components available to all iGEM teams so anyone could easily produce this system for cell-free protein expression. In total there are 38 TX-TL proteins that we would like to register as a parts collection.

This collection will grow as we continue to develop a tRNA and ribosome purification strategy in BioBrick standard as well.

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  • To add to this list, please use the category //collections/cell-free_tx-tl