Composite

Part:BBa_K2262015

Designed by: Yi-Ting Lin, Huei-Yu Yeh, Sheng-Ping Chu   Group: iGEM17_NCTU_Formosa   (2017-10-24)
Revision as of 17:22, 24 October 2017 by Registry (Talk | contribs)

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Constitutive Promoter + RBS + fMt

This sequence is designed for constitutively chelating arsenic ions. We ligated a constitutive promoter(BBa_J23119) with metallothionein (fMT, BBa_K190019) to produce arsenic-binding protein. This metallothionein (fMT) is a kind of chelating protein from Fucus vesiculosus. It can bind both Arsenite (III) and Arsenate(V). This part was first designed by Groningen of iGEM 2009. The part of K190019 consists of fMT and RBS(ribosome--‐binding Site, for initiating translation).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 170
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None