Composite

Part:BBa_K2224001:Experience

Designed by: Yunchang Zhang   Group: iGEM17_SMS_Shenzhen   (2017-10-24)
Revision as of 03:30, 28 October 2017 by Jeff Du (Talk | contribs) (→‎Applications of BBa_K2224001)


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Applications of BBa_K2224001

Part Description:

BBa_K2224001 is a composite part with the backbone pSB1C3. The CDS of this part is a chitinase originate from verticillium lecanii, a kind of entomogenous fungus. With a GeneBank identifier DQ412944 in NCBI. This enzyme helps catalyze chitin. SMS students optimized the codons and removed inrtons to fit the original sequence into E. coli expression system.

Experiment Abstract

The original sequence DQ412944 was published on NCPI. SMS students have processed the CDS. Designed primer with restriction sites, modified the CDS for standardization. We then synthesized this plasmid with the backbone pSB1C3. Enzyme activity in TOP10 E. coli expression system related to time shown below. Chitinase Activity.png

User Reviews

UNIQfa3205b34ca3b0d2-partinfo-00000000-QINU UNIQfa3205b34ca3b0d2-partinfo-00000001-QINU