Reporter

Part:BBa_K1943013:Design

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-10-13)
Revision as of 03:15, 22 October 2016 by John Tsq (Talk | contribs) (→‎Source)


Puro


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 481


Design Notes

This year we use mammalian cells as carriers. So in our plasmids, there are several kinds of anti-antibiotics genes which are used for screening. We design primers and do PCR of this anti-antibiotics genes coding sequence and ligate it to pSB1C3 backbone.

Source

The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.

References