Part:BBa_K2075004
Vector iGem_02_Ax7L- DS
This part synthesize a transcription activator like (TAL) effector protein. They were found in Xanthomonas bacteria. These bacteria are plant pathogenes and infects plants via a secretion typ III system. In the plant cells these proteins binds promoter sites. This leads to a transcriptional activation of the genes behind the promoter. The proteins contains tandem repeats up to 34.5. Each repeat binds one nucleotide of the target sequenz. The 12. and 13. Aminoacid of each repeat leads to the specific binding of the DNA sequence.
We circularised the TAL-effectors with the help of Inteins at the N- and C- Terminus of each TAL-effector.
The 12. And 13. Aminoacid of each of the 11.5 repeats from our TAL Hax3 – 2xNN are: NI NN HD NI HD NG NI NG NI NG NI NI NI HD HD HD HD HD They bind the DNA sequence: A G/A C A C T A T A T A A A C C C C C
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3370
Illegal BamHI site found at 2776 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2715
Illegal NgoMIV site found at 3310 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2790
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