Composite
Part:BBa_K1897023
Designed by: Keshiniy Madivannan Group: iGEM16_NUS_Singapore (2016-10-11)
p62-32-ALR-Terminator
The construct contains a wild-type lldPRD operon promoter from E. coli (Part:BBa_K822000) with a medium RBS flanked by non-coding sequences (Part:BBa_K1897032), followed by a CDS of ALR (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 501
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 425
Illegal BamHI site found at 1127 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 543
Illegal AgeI site found at 843 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 300
[edit]
Categories
Parameters
None |