Composite

Part:BBa_K1899005:Experience

Designed by: iGEM2016_HKUST   Group: iGEM16_Hong_Kong_HKUST   (2016-10-10)
Revision as of 14:57, 17 October 2016 by Leona (Talk | contribs)


Applications of BBa_K1899005

748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg

Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240 and pSB3K3-PhlFp- BBa_E0240

The fold change between pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240) and that of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240 is around 13.2 times and 7.01 times respectively.

This is due to the toxicity of <bbcode>BBa_C0040 <bbcode>(TetR). The toxicity reduces the growth rate of the E. coli containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say TetR interferes the functionality of PhlFp at this stage.

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