Composite
Part:BBa_K1897021
Designed by: Keshiniy Madivannan Group: iGEM16_NUS_Singapore (2016-10-11)
p70-33-ALR-Terminator
The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 (Part:BBa_K1847008) with a weaker RBS flanked by non-coding sequences (Part:BBa_K1897031), followed by a CDS of ALR (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101
Illegal NheI site found at 499 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 423
Illegal BamHI site found at 1125 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 541
Illegal AgeI site found at 841 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 298
[edit]
Categories
Parameters
None |