Translational_Unit

Part:BBa_K1968015

Designed by: Alejandra Adriana Schiavon Osorio   Group: iGEM16_Edinburgh_OG   (2016-10-13)
Revision as of 19:12, 13 October 2016 by Registry (Talk | contribs)

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Phleomycin resistance cassette Phytobrick

Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, is widely used as a selection marker for transformation. The device is designed for filamentous fungi and is controlled by the PgdaA, a constitutive promoter and the Tcyc terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 379
    Illegal NgoMIV site found at 440
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None