Generator

Part:BBa_K2012016

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-10-06)
Revision as of 04:27, 7 October 2016 by Registry (Talk | contribs)

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A weak CheZ generator with fusion GFP reporter

This generator contain promotor J23117 ,RBS from BBa_J61100,functional chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate weak concentration of CheZ which can be reported by the fluorescence intensity.It is used to detect the relationship of CheZ under different strength of promotor and its swimming ability

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362


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Parameters
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