Part:BBa_K1951005
FliC E.coli coding sequence
This coding sequence codes the Flagellin C (FliC) protein, filament of the flagellum from Escherichia coli strain.
Flagellin is the main subunit protein which polymerizes to form the filaments of bacterial flagella and is involved to promote bacterial swimming. In the possession of flagellum, bacteria can move what confers them a selective advantage. [1]
It has been demonstrated that Flagellin has the ability to adsorbe precious metal such as Platinum, gold..
Design summary
Starting from the E.coli flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in E. coli chassis. All forbidden restriction sites have been removed. Codons have been optimized for E.coli to allow a maximal transcription level.
Proof of fonctionnality
This sequence has been tested under the control of a strong promoter and validated as fonctionnal. For further information, go on the following link : BBa_K1951008 design
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1224
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 709 - 1000COMPATIBLE WITH RFC[1000]
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