Regulatory

Part:BBa_K2165004:Design

Designed by: Roya Amini-Naieni   Group: iGEM16_Washington   (2016-10-14)
Revision as of 02:49, 15 October 2016 by Registry (Talk | contribs)

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Violacein C gene with CUP1 promoter + ADH1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 370


Design Notes

The Yeast Promoter Atlas was used to prevent altering bases that served a significant regulatory purpose (http://ypa.csbb.ntu.edu.tw/do?act=gene_by_kw&query=YHR053C#promoter_map). One base pair was substituted to eliminate the XbaI site in the middle of the sequence.


Source

The CUP1 promoter was identified in the 5' untranslated region of the metallothionein gene in Saccharomyces cerevisiae.

References