Regulatory

Part:BBa_K2151000:Design

Designed by: Matej Vucak, Katy Baker, James Provan   Group: iGEM16_Glasgow   (2016-08-30)
Revision as of 18:19, 8 October 2016 by Mvucak (Talk | contribs) (→‎Design Notes)


pHLBA, strong constitutive S. thermophilus promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was not altered for use in E. coli as the aim was to achieve expression in S. thermophilus

Source

  • Sequence obtained from 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene'.
  • Synthesized by IDT as an oligo-nucleotide

References

Chouayekh, H., Serror, P., Boudebbouze, S. and Maguin, E. (2009) 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene', Fems Microbiology Letters, 293(2), pp. 232-239.