Coding

Part:BBa_K1957001:Design

Designed by: Nancy Teng   Group: iGEM16_NRP-UEA-Norwich   (2016-10-06)
Revision as of 18:57, 8 October 2016 by Nteng (Talk | contribs) (Origin of replication details)


HydC subunit of FeFe Hydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 700


Design Notes

This is one of of the three FeFe Hydrogenase subunits. To make the entire FeFe Hydrogenase construct the following BioBricks need to be ligated with BBa_K1957001: BBa_K1957004 with BBa_K1957002 or BBa_K1957004 with BBa_K1957003.

BBa_J04450, which contained pSB1C3 would not conjugate properly into S.oneidensis, most likely because conflicts in the origin of replication. To overcome this, the three subunits were ligated using golden gate cloning into the pBAD expression vector.

Source

The source of the part will be its sequence which was retrieved from GenBank.

References