Coding

Part:BBa_K2065001

Designed by: Tom van Sonsbeek   Group: iGEM16_TU-Eindhoven   (2016-09-26)
Revision as of 11:34, 26 September 2016 by Registry (Talk | contribs)

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CT52-mNeonGreen

CT52 is linked to mNeonGreen by a flexible GGS-10 linker to make sure the mNeonGreen is able to move towards other bound proteins. This insert is placed into a pET28a vector and expressed by the E. Coli BL21 (DE3) bacteria. The part is used for BRET assays in the tetrameric scaffold together with the CT52-SsNL and CT52-LsNL. mNeonGreen-CT52 will be the acceptor that changes the wavelength of the light emitted by NanoLuc to a wavelength of 517 nm. The sequence of our CT52-mNeonGreen has been verified by Starseq. It contains the prefix and suffix with the correct restriction sites (EcoRI, XbaI, SpeI and PstI). mNeonGreen-CT52 is 956 bp long.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 964
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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