Coding

Part:BBa_K1919000:Design

Designed by: Shiji Zhao   Group: iGEM16_SCU-China   (2016-09-04)
Revision as of 14:41, 4 September 2016 by Century (Talk | contribs) (→‎References)


The coding sequence of antimicrobial peptide CecropinXJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence was optimized for E.coli expression using JCat http://www.jcat.de/. The single stop codon was replaced by double TAA stop codon.


Source

The original sequence of CecropinXJ comes from GenBank NM_001043995.1.

References

Xia L, Zhang F, Liu Z, et al. Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli[J]. Experimental & Therapeutic Medicine, 2013, 5(6):1745-1751.