Reptorter proteins
Fluorescent proteins
| Fluorescent proteins
|
| Protein
|
Color
|
BioBrick
|
Excitation [nm]
|
Emission [nm]
|
Quantum Yield
|
| Cerulean
|
Cyan
|
BBa_I757009
|
433
|
475
|
0.62
|
| GFP
|
Green
|
BBa_K648013
|
484
|
507
|
0.60
|
| GFP
|
Green
|
BBa_K863120
|
484
|
507
|
0.60
|
| GFP
|
Green
|
BBa_K823039
|
484
|
507
|
0.60
|
| sfGFP
|
Green
|
BBa_K1321337
|
485
|
510
|
0.65
|
| Dronpa (photoactivatable)
|
Green
|
BBa_K1680006
|
503
|
518
|
0.85
|
| mVenus
|
Yellow
|
BBa_I757008
|
515
|
528
|
0.57
|
| sYFP2
|
Yellow
|
BBa_K1159301
|
514
|
527
|
0.61
|
| eCFP
|
Cyan
|
BBa_K1159302
|
439
|
476
|
0.40
|
| mRFP
|
Red
|
BBa_K1351021
|
584
|
607
|
0.25
|
| mRFP
|
Red
|
BBa_K1615089
|
584
|
607
|
0.25
|
| mKate2
|
Red
|
BBa_K823029
|
588
|
633
|
|
Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.
Luciferases
| Luciferases
|
| Protein
|
BioBrick
|
Size [kDa]
|
| Firefly luciferase
|
BBa_K525999
|
61
|
| Renilla luciferase
|
|
36
|
| NanoLuc luciferase
|
BBa_K1159001
|
19
|
| Gaussia luciferase
|
|
|
Transmembrane Domains
Linker domains
| Linker
|
| Protein
|
Sequence
|
BioBrick
|
Length [residues]
|
| Short Linker
|
(Gly-Gly-Ser-Gly)
|
BBa_K243004
|
4
|
| Middle Linker
|
2x (Gly-Gly-Ser-Gly)
|
BBa_K243005
|
8
|
| Long Linker
|
3x (Gly-Gly-Ser-Gly)
|
BBa_K243006
|
12
|
|
|
|
|
|
|
|
|
|
|
Catalytic domains
| Fluorescent proteins
|
| Erythromycin Esterase Type II
|
BBa_K1159000
|
| Laccase
|
BBa_K1159002
|
| Laccase TVEL5
|
BBa_K1615067
|
| TEV Protease
|
BBa_K1159100
|
| Thermonuclease
|
BBa_K1159105
|
| Heroin Esterase
|
BBa_K1615045
|
| CRE recombinase
|
BBa_K1680007
|
| Monoamine Oxidase A
|
BBa_K1615022
|
| Endo-β-Galactosidase
|
BBa_K1483001
|
| α-Galactosidase
|
BBa_K1483002
|
| Acetylgalactosamindase
|
BBa_K1483000
|
| 2-oxoglutarate oxygenase/decarboxylase
|
BBa_K1065000
|
Transcription Regulators
| Fluorescent proteins
|
| VP16
|
BBa_K1150001
|
Split proteins
| Fluorescent proteins
|
| TEV Protease
|
BBa_K1159101
|
BBa_K1159102
|
| SpyCatcher/SpyTag
|
BBa_K1159200
|
BBa_K1159201
|
Binding proteins
| Fluorescent proteins
|
| Fluorescein binding Anticalin
|
BBa_K1159003
|
| Digoxigenin binding Anticalin
|
BBa_K243003
|
| HER2 binding DARPin
|
BBa_K404302
|
| HER2 binding Affibody
|
BBa_K404302
|
| Nickel Binding Protein
|
BBa_K1321009
|
| Cellulose binding Domain
|
BBa_K863110
|
Affinity tags
| Affinity tags
|
| HA tag
|
BBa_K1150016
|
| His6-tag
|
BBa_I757013
|
| SpyTag
|
BBa_K1159020
|
| Strep-tag II as N-part
|
BBa_K549018
|
| Strep-tag II
|
BBa_J18916
|
| cMyc-tag
|
BBa_K823036
|
| 3x FLAG tag
|
BBa_K823034
|
