Part:BBa_K1840008
Device: TT-gad-mCherry
This device acts as a detector for glucose (and certain derivatives of it). The double transcription terminator ensures, that only the gene downstream of the gad promotor is influenced by it. The promotor (BBa_K1840003) is contained in the genome of Pseudomonas putida and is situated in front of the enzyme gad (gluconate oxidase). The repressor PtxS can bind the gad promotor sequence and thus prevent the transcription of the gad gene. In case the glucose derivative 2-ketogluconate is present, it binds the promotor and leads to a subsequent conformational change. Hence, the repressor can no longer bind and the gene downstream can be transcribed. In our device, the downstream gene is mCherry, codon optimized for Pseudomonas (BBa_K1840004). Therefore, in the presence of glucose, mCherry is expressed. In various experiments, we could see that this device is in deed working.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 325
- 1000COMPATIBLE WITH RFC[1000]
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