Generator
Part:BBa_K1859021:Experience
Designed by: Yusuke Banno Group: iGEM15_Gifu (2015-09-09)
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how you used this part and how it worked out.
===Applications of BBa_K1859021===
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane F shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no fluorescence. We found that this generator doesn’t make functional long chain protein.
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