RNA

Part:BBa_K1614020

Designed by: Daniel Heid   Group: iGEM15_Heidelberg   (2015-09-18)
Revision as of 14:14, 20 September 2015 by Frieda (Talk | contribs)

ATP Aptamer JAWS2 Spinach 2.1

The ATP Aptamer JAWS 2.2 is cloned into the RFC 110 and can be transcribed using the T7 RNA Polymerase. This BioBrick can be used as a tool for sensing ATP in vivo.

Usage and Biology

ATP Aptamer JAWS2 Spinach 2.1 is a fusion of the BBa_K1330000 generated by DTU Danemark. According to our results, this fusion should contain similar properties as BBa_K1614014. Yet, this Spinach 2.1 is ligand dependend to ATP and should find its use in the detection of the small molecule ATP. Applications such as nucleotide sensing during in vitro transcription or because of its stronger stem for live cell imaging are possible. For more information check BBa_K1614014 or BBa_K1614015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None