Coding
Biosurfact
Part:BBa_K653000:Experience
Designed by: Team Panama 2011 Group: iGEM11_Panama (2011-09-26)
Revision as of 19:05, 16 September 2015 by RAKemal (Talk | contribs) (→Applications of BBa_K653000)
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how you used this part and how it worked out.
Applications of BBa_K653000
Experience of the ITB_Indonesia iGEM 2015 team.
The iGEM 2015 ITB Indonesia used this coding sequence under T7lac promoter and RBS (BBa_K613010) with a double terminator (BBa_B0015) for rhamnolipid production in E. coli BL21(DE3). After IPTG induction, the separated supernatant was tested for surfactant activity.Supernatant from empty cell, either induced or not, had no / little surfactant activity. The uninduced transformant had little activity, but the induced transformant had higher surfactant activity.
This shows that the part was induced for enzyme expression and biosurfactant production. To our knowledge, this is the very first time that the part was used for expression.
User Reviews
UNIQ195ca692ff5b35e6-partinfo-00000001-QINU UNIQ195ca692ff5b35e6-partinfo-00000002-QINU