Reporter

Part:BBa_K1734006

Designed by: Eduardo Ramírez Montiel   Group: iGEM15_Tec-Monterrey   (2015-09-18)
Revision as of 09:51, 18 September 2015 by Edu92ram (Talk | contribs) (Created page with "This part encodes a sequence capable to add a purification tag (6X His) at the 3’ terminus of a desired protein. Furthermore, by using the reporter gene (nanoluc) it’s possib...")

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This part encodes a sequence capable to add a purification tag (6X His) at the 3’ terminus of a desired protein. Furthermore, by using the reporter gene (nanoluc) it’s possible to quantify indirectly the protein concentration. The T2A sequence cleaves the protein [1], producing the protein of interest tagged with HisTag and nanoluc separately This part has been codon optimized for Spodoptera frugiperda (Sf9)


Reference: [1] Kim JH, Lee S-R, Li L-H, Park H-J, Park J-H, Lee KY, et al. (2011) High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice. PLoS ONE 6(4): e18556. doi:10.1371/journal.pone.0018556

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