Generator

Part:BBa_K1833008

Designed by: Konstantin Borisov   Group: iGEM15_Pitt   (2015-09-17)
Revision as of 04:55, 18 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pT7-MMP9 sensitive repressor

This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 9, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on this part, visit 2015.igem.org/Team:Pitt/Protease/Project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 297
    Illegal NgoMIV site found at 696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 194
    Illegal SapI.rc site found at 53


[edit]
Categories
Parameters
None