IGEM:Bordeaux/2015/stationary phase curdlan synthase

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[[800px-Bordeaux_Team_promoter_characterizationV4.png

Curdlan synthase under the expression of stationary phase promoter

To demonstrate growth phase dependent activation of our Curdlan synthase gene, we compared the amount of Curdlan produced by E.coli while at the same time looking at OD to measure growth. The Curdlan concentration was calculated with absorption measures (excitation wavelength at 398nm and emission wavelength= 502nm) after Curdlan purification from cells. We used aniline blue dye since it is specific of beta glucans.]]