Part:BBa_K1755001
ribB coding sequence
ribB;the 3,4 dihydroxy-2-butanone-4-phosphate synthase;
We get this part from E.coli genome by synthetize. It can increase the electric output by synthesis of riboflavin.
Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow.
Contents
Usage in MFC
Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications, because of the electric output is too low. Riboflavin is an excellent electron transport complex. However, the whole sequence of riboflavin synthase is too huge(more than 3000bp) to link to the plasmid pSB1C3. Evidence shows that, part of the riboflavin synthase DNA sequence (ribB) can also improve the secretion of riboflavin.
Usage and Biology
The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.
Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
Catalytic activity
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.
Sequence (amino acid)
10 20 30 40 50 60 70 80 90 100
TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM
110 120 130 140 150 160 170 180 190 200
GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR
210
TIEDLVAYRQ AHERKAS
Figure 1:Normal E.coli(left one)and E.coli with ribB(right one)
Figure 2:Constitutive parts riboflavin expression level
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 387
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 319 - 1000COMPATIBLE WITH RFC[1000]
references
1.UniPort P0A7J0 RIBB_ECOLI [http://www.uniprot.org/uniprot/P0A7J0]
2.iGEM 2014 LZU-China Part:BBa_K1523101 [1]
3.Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli.Richter G., Volk R., Krieger C., Laham H.-W., Roethlisberger U., Bacher A.J. Bacteriol. 174:4050-4056(1991）
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