File:2015 Freiburg SDS-PAGE 13 15.png
Revision as of 09:06, 31 August 2015 by RamonaE (Talk | contribs) (12,5% SDS-PAGE analysis of the protein purification of S. Typhimurium antigen (dehydroxyacid dehydratase) and S. Typhimurium antibody (anti dehydroxyacid dehydratase). The protein purification was performed with gravity flow columns and Ni-NTA Agarose. Th)
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12,5% SDS-PAGE analysis of the protein purification of S. Typhimurium antigen (dehydroxyacid dehydratase) and S. Typhimurium antibody (anti dehydroxyacid dehydratase). The protein purification was performed with gravity flow columns and Ni-NTA Agarose. The protein was eluated by 500mM Imidazole. The expected molecular weight for the S. Typhimurium antigen is 63 kDa and 37 kDa for the antibody, respectively. FT-Flowthrough, W-Wash, E-Elution(Meyer et al., 2012)
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current | 12:35, 14 September 2015 | 1,341 × 875 (910 KB) | RamonaE (Talk | contribs) | Reverted to version as of 12:27, 14 September 2015 | |
12:29, 14 September 2015 | 1,341 × 875 (910 KB) | RamonaE (Talk | contribs) | |||
12:27, 14 September 2015 | 1,341 × 875 (910 KB) | RamonaE (Talk | contribs) | |||
09:06, 31 August 2015 | 1,223 × 876 (611 KB) | RamonaE (Talk | contribs) | 12,5% SDS-PAGE analysis of the protein purification of S. Typhimurium antigen (dehydroxyacid dehydratase) and S. Typhimurium antibody (anti dehydroxyacid dehydratase). The protein purification was performed with gravity flow columns and Ni-NTA Agarose. Th |
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