Help:Submission Kit

Registry Help Pages:

TOC
At-a-Glance
FAQ

DNA Submission

Sample submission of parts is no longer a requirement for the iGEM competition.

Requirements

Here's how part samples are sent to the Registry

Why pSB1C3?

Why do samples need to be shipped in pSB1C3?

Detailed Instructions

Instructions on how to fill out the Registry's submission form.

Submission Prep

Registry groups must follow iGEM's submission prep guidelines.

Have questions on requirements or shipping part samples? Check the FAQ or contact us at hq (at) igem . org .

For iGEM 2015, we are standardizing the part submission process.

As in previous years, parts must be BioBrick compatible and in pSB1C3. Additionally, all part submissions must be shipped as dried DNA, in 96 well plate format, and covered with adhesive foil.

We've provided a submission kit with all of the materials you'll need to ship your parts.

(3) 96-well skirted PCR plates
(3) universal plate lids
(5) adhesive foils
bubble wrap
a box with the iGEM HQ address, for shipping your submission

Sample Layout

Make sure to add your samples in the correct layout:

Your first sample (s1) should be dispensed into well 1A of your submission plate.
Your second sample (s2) should be dispensed into well 1B of the same submission plate.

Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.

Sample DNA Quantity

250ng minimum of purified plasmid DNA (your part in pSB1C3)
DNA must be dried down before shipping

We recommend drying down the plate in your lab's laminar flow hood.

Sterilize the hood surface with 70% Ethanol
Cover the 96 well plate with the provided plate lid (not with the adhesive foil)
Place the plate in the hood.
With sufficient airflow, drying down 10ul (25ng/ul) of purified plasmid DNA in the plate should take four hours.
Once all wells have dried, cover the plate with the provided adhesive foil.

Plate Labelling

Make sure to label each plate with the appropriate submission number.

Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.