Coding

Part:BBa_K1321354

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 18:18, 17 October 2014 by Xen sm (Talk | contribs)

sfGFP fused to CBDclos driven by T7

A T7-promoter expression construct of super-folder GFP fused N-terminally to CBDclos (a cellulose-binding domain).

This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: IC14-sfGFP-part-table.PNG

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 68


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Categories
Parameters
None