Coding

Part:BBa_K1331006

Designed by: Tianyi Huang   Group: iGEM14_Nankai   (2014-09-26)
Revision as of 04:10, 19 October 2014 by Danielle D (Talk | contribs) (→‎Usage and Biology)

Modified rhlAB coding sequence from Pseudomonas aeruginosa SQ6


This part encodes both the subunit A and B of rhamnosyltransferase I. It is composited with BBa_K1331001 and BBa_K1331004.

The part includes both the coding sequence of RhlA, RhlB and the whole noncoding sequence between RhlA and RhlB.

The purpose of remaining this noncoding sequence is to ensure the normal expression of rhamnosyltransferase I.

Usage and Biology

This part encodes both the subunit A and B of rhamnosyltransferase I, which is the essential protein for rhamnolipid synthesis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
    Illegal XhoI site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 992
    Illegal NgoMIV site found at 1713
    Illegal NgoMIV site found at 1826
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI site found at 1342
    Illegal BsaI.rc site found at 478


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
None