Reporter

Part:BBa_K1431812:Design

Designed by: Yicong Tao, Yushan Zhang   Group: iGEM14_SUSTC-Shenzhen   (2014-10-14)
Revision as of 22:29, 17 October 2014 by Majia67 (Talk | contribs) (→‎Design Notes)

amilCP, blue chromoprotein reporter system (Strong Promoter, Strong RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This Biobrick is constructed through 3 steps:

Step# Do double enzyme digestion (XbaI & PstI for BBa_K592009, SpeI & PstI for BBa_B0034) and do gel extraction and ligation. Step#

Source

The following list is the Biobricks we've used and their detailed design information.

Part Type Discription Backbone Location on the plate
BBa_J23100 Promoter Strong Promoter BBa_J61002 (Amp) 17D 2014 Kit Plate 4
BBa_J23106 Promoter Weak Promoter BBa_J61002 (Amp) 17P 2014 Kit Plate 4
BBa_B0031 RBS Weak RBS pSB1A2 1H 2014 Kit Plate 4
BBa_B0034 RBS Strong RBS pSB1A2 1N 2014 Kit Plate 4
BBa_B0015 Terminator Strong terminator (B0010+B0012) pSB1C3 3F 2014 Kit Plate 3
BBa_K592009 Chromoprotein amilCP, blue chromoprotein pSB1C3 19E 2014 Kit Plate 1
BBa_K592011 Chromoprotein cjBlue, green chromoprotein pSB1C3 2I 2014 Kit Plate 4
BBa_K1033916 Chromoprotein amajLime, yellow-green chromoprote pSB1C3 6M 2014 Kit Plate 4

References