Composite

Part:BBa_K1433008

Designed by: Hanqing Liu   Group: iGEM14_ZJU-China   (2014-09-28)
Revision as of 16:45, 28 September 2014 by Registry (Talk | contribs)

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6N-gp35-Terminator

Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination between attB and attP, the attachment sites on the phage chromosome and host chromosome. This recombination result in reverse of the sequence between attB and attP, then form new sites attL and attR. This revert can restore by appropriately controlling the conditional heterologous expression of integrase and an excisionase in Bxb1 named gp47. This part is composed of 3 elements. 1. RBS: 6N, a suitable RBS in paper.
 2. gp35: a serine integrase in Mycobacterium phage Bxb1. 3. terminator: BBa_B0015, a strong double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
    Illegal NheI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 491
    Illegal XhoI site found at 578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1130
    Illegal NgoMIV site found at 1217
    Illegal AgeI site found at 267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1325


[edit]
Categories
Parameters
None