Part:BBa_K1463000

Recombinase Switch With GFP and RFP

A recombinase switch with GFP one side of the switch and RFP on the other.

thumb|500px|right| Fig. 1: 1.pZJ7 on its own, 2.Switch #2 on its own, 3.Switch #2 + pZJ7 glucose, 4.Switch #2 + pZJ7 arabinose, 5.Switch #3 on its own, 6.Switch #3 + pZJ7 glucose, 7.Switch #3 + pZJ7 arabinose, 8.Switch #4 on its own, 9.Switch #4 + pZJ7 glucose, 10.Switch #4 + pZJ7 arabinose, 11.pBAD33 gvpAC (ignore)12.1kb+ marker.

Fig. 1: 1.pZJ7 on its own, 2.Switch #2 on its own, 3.Switch #2 + pZJ7 glucose, 4.Switch #2 + pZJ7 arabinose, 5.Switch #3 on its own, 6.Switch #3 + pZJ7 glucose, 7.Switch #3 + pZJ7 arabinose, 8.Switch #4 on its own, 9.Switch #4 + pZJ7 glucose, 10.Switch #4 + pZJ7 arabinose, 11.pBAD33 gvpAC (ignore)12.1kb+ marker.

The image below shows a schematic of the switch. The

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 813
Illegal NheI site found at 836
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 793
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 34
Illegal AgeI site found at 146
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 711
Illegal BsaI.rc site found at 1668