Composite
Part:BBa_K1497007:Design
Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen Group: iGEM14_TU_Darmstadt (2014-10-07)
Naringenin producing operon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1104
Illegal NheI site found at 4631 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1671
Illegal BglII site found at 4640
Illegal XhoI site found at 3638 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1777
Illegal NgoMIV site found at 2609
Illegal NgoMIV site found at 4611
Illegal NgoMIV site found at 5187
Illegal AgeI site found at 1872
Illegal AgeI site found at 2038 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3067
Illegal BsaI site found at 4560
Illegal BsaI.rc site found at 1303
Illegal BsaI.rc site found at 4042
Design Notes
no
Source
The functionality of this construct is verified by putting the device under a promotor. Proofing functionality leaded to Bba_K1497008 (Naringenin Operon under the constitutive promotor J23100) and Bba_K1497008 (Naringenin Operon under the T7 Promotor).