Coding
REACh2

Part:BBa_K1319002

Designed by: Michael Osthege, Florian Gohr   Group: iGEM14_Aachen   (2014-09-30)
Revision as of 12:57, 30 September 2014 by Registry (Talk | contribs)

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RFC[25]-compatible dark quencher based on K1319000 (E0030)

This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP).

Three mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W
  • H148R

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None