Composite

Part:BBa_K1520515

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-23)
Revision as of 03:10, 24 September 2014 by Registry (Talk | contribs)

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MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter.

MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter. It is a test of our positive feedback circuit for microcystin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 954
    Illegal NheI site found at 977
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 796
    Illegal BglII site found at 2798
    Illegal BglII site found at 3468
    Illegal XhoI site found at 2770
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1932


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controlluxR, HSL
directionForward
ec_numnone
functionMixed
keggnone
ligandsHSL
n/aconstitutive promoter family member
negative_regulators
o_h
o_l
positive_regulators1
proteinLuxR
swissproP12746
tagNone
uniprotP48445