Composite

Part:BBa_K1298003:Design

Designed by: CoBRA Team   Group: iGEM14_CoBRA   (2014-06-17)
Revision as of 23:28, 17 June 2014 by Autumnbernard (Talk | contribs) (→‎Source)


PcChia 1-1 (insert) using J04500


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 745
    Illegal AgeI site found at 671
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 229


Design Notes

With the PcChia 1-1 protein code, illegal cut sites were removed using DNA optimization. There is also no secretory tag; the chitinase protein being produced is not being secreted outside of the cell, but staying inside.


Source

BBa_K1298000 (PcChia 1-1)

  • Phytochemistry paper of Dr. Kolosova (1)
  • NCBI (protein code) (2)
  • Bio Basic Inc. (DNA optimization)

References

1. Kolosova, N. “Cloning and characterization of chitinases from interior spruce and lodgepole pine.” 1 Oct. 2013. Feb. 2014 <http://www.ncbi.nlm.nih.gov/pubmed/24564978>

2. Kolosova, N. "Picea engelmannii x Picea glauca class I chitinase (Chia1-1) mRNA, complete cds." 30 Dec. 2013. Feb. 2014 <http://www.ncbi.nlm.nih.gov/nuccore/HM219844>*

*The sequence found at this link has been optimized to remove illegal cut sites (EcoRI, XbaI, SpeI, PstI) and therefore is not the same as the sequence found on its part page.