Composite

Part:BBa_K1298003:Design

Designed by: CoBRA Team   Group: iGEM14_CoBRA   (2014-06-17)
Revision as of 23:21, 17 June 2014 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PcChia 1-1 (insert) using J04500


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 745
    Illegal AgeI site found at 671
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 229


Design Notes

With the PcChia 1-1 protein code, illegal cut sites were removed using DNA optimization. There is also no secretory tag; the chitinase protein being produced is not being secreted outside of the cell, but staying inside.


Source

BBa_K1298000 (PcChia 1-1) : Phytochemistry paper of Dr. Kolosova (1), NCBI (protein code) (2), Bio Basic Inc. (DNA optimization)

References