Regulatory

Part:BBa_K1045004:Design

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)
Revision as of 14:01, 26 October 2013 by Kati (Talk | contribs) (→‎References)

YdaO Riboswitch with native Promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into EcoRI and PstI digested pSBC13 backbone.

Source

This part was generated by PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG StĂŒlke, Department for General Microbiology, University Göttingen).

References

(1) Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965

(2) James W Nelson, Narasimhan Sudarsan, Kazuhiro Furukawa, Zasha Weinberg, Joy X Wang & Ronald R Breaker (2013) “Riboswitches in eubacteria sense the second messenger c-di-AMP“, Nature Chemical Biology, Advance Online Publication [doi: 10.1038/nchembio.1363]