Generator

Part:BBa_K1060011

Designed by: Ingmar Claes, Lukas Marcelis, Su Wang   Group: iGEM13_KU_Leuven   (2013-09-19)
Revision as of 14:15, 4 October 2013 by Dinox (Talk | contribs)

Beta Farnesene production

For the production of the alarmhormone, (E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006). Additionally a Lac operator sequence (Gilbert & Maxam, 1973) was introduced to regulate its expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 209
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1409
    Illegal BamHI site found at 709
    Illegal XhoI site found at 671
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 281

BBa_K10600011_gel_pic.jpg


[edit]
Categories
Parameters
None