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ccdB_Ind

Part:BBa_K1152007:Experience

Designed by: Joshua Sachs   Group: iGEM13_Heidelberg   (2013-09-20)
Revision as of 10:43, 4 October 2013 by Joshua (Talk | contribs)

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Applications of BBa_K1152007

pJS01 was amplified out of four fragments via Gibson Cloning: ccdB, the C-domain of TycC2 and the indC-gene, as well as a backbone. We verified the genotype by colony-PCR, restriction digest with various restriction enzymes, among them EcoRI and PstI, and sequencing of the assembly overhang regions. One part of the standard, we propose the possibility to tag Non-Ribosomal Peptides with Indigoidine. Using this helper-construct eased our work in bringing the proof of principle for the functionality of the Indigoidine-tag, as by replacing the ccdB with the NRPS modules of interest, re-ligation background is significantly decreased. This construct stands as representative of the various fusion-peptides with Indigoidine-tag that we synthesized during our project.

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UNIQ278ffe1aff53caec-partinfo-00000000-QINU UNIQ278ffe1aff53caec-partinfo-00000001-QINU