PBAD SPL

Description

This is a page containing a synthetic promoter library (SPL) of pBAD promoters made by [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team]. The library can be used to make and arabinose inducible system with very low leakiness, or tune the the promoter strength of the system when it's turned on.

A tight inducible system can be a very useful tool to express lethal or growth inhibiting proteins in E.coli. It can also be necessary with a non leaky expression system if you would like to visualize the expression of GFP SF in E.coli (see [http://2013.igem.org/Team:DTU-Denmark/HelloWorld link]).

The library

Here is a complete list of the sequences and their relative leakiness and strength:

Identifier	Sequencea	Induced Strength	Leakyness	Inducibilityb
Col. 2	CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA	14.2	0.203	69.9
Col. 3	CTGACGTAATCATACCGCCGAAAGTATTATCATTA	16.6	0.426	39
Col. 4	CTGACGCGACAACATTGCGTCCTATAAAATGCCGA	14.2	0.345	41
Col. 5	CTGACGGCCCGCCCCCGATGCGCATAAAATACCAA	17.7	0.424	41.6
Col. 12	CTGACGCGTATCGCGAGCGGGCGTTATTATACGCA	13.6	0.414	32.9
Col. 10	CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA	18	0.462	38.8
Col. 9	CTGACGCCACCCCCCCCCCCGGCGTATAATTCCCA	16.1	0.530	30.4
Col. 8	CTGACGCCCTACCGCTCGCCCCCCTATTATACCCA	13.4	0.211	63.4
Col. 13	CTGACGCCCCCCCCGTCCACCCCCTAATATCCCGA	10.9	0.096	113.6
Col. 15	CTGACGTGCGCCTGCCGTCCAAAGTAATATCCTTA	15.5	0.235	65.8
Col. 18	CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA	19.8	0.524	37.8
Col. 19	CTGACGCTCGCACCGGCGACCCGATAATATCCATA	18.0	0.315	57
Col. 31	CTGACGCCCCCCCTCCCCCGACCTAAAATATCCAG	3.8	0.161	23.6
Col. 29	CTGACGCCGCTGCACCCGTCCCCCTATTATAGCAA	14.8	0.301	49.2
Col. 26	CTGACGCCGATCCTATCTCCCTATTAAAATTTTTA	13.8	0.292	47.2
Col. 22	CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA	16.8	0.466	36.1
Col. 33	CTGACGCCAGCTTCCTACTTCAAATATAATCCGTA	14.4	1.01	14.3
Col. 34	CTGACGTCCCTCTTCCGCGCCCCCTAAAATACCCA	12.4	0.187	65.9
Col. 35	CTGACGCCCAACCCGACACAGCGATATAATAATGA	18.4	0.89	20.7

^a blabla ^b blablablabla